Lipid production by Yarrowia lipolytica growing on industrial glycerol

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Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture

Seraphim Papanikolaou and George Aggelis,

Laboratory of General and Agricultural Microbiology, Department of Agricultural Biotechnology, Agricultural University of Athens, 75 Iera Odos, 11855 Votanikos, Athens, Greece - submitted year: 2002

Abstract

Yarrowia lipolytica LGAM S(7)1 presented remarkable growth on industrial glycerol used as sole carbon substrate. Nitrogen-limited flask cultures were accompanied by restricted synthesis of reserve lipid, whilst amounts of citric acid were produced extracellularly. On the contrary, high amounts of reserve lipid (up to 3.5 g/l, 43% w/w of lipids in dry biomass) were produced in highly aerated continuous cultures. Lipid production was favoured at low specific dilution rates whilst fat-free material yield increased over the whole range of D (h?1). The maximum volumetric productivity obtained was 0.12 g lipid/l h. Storage lipid composition did not present remarkable changes in the specific dilution rates tested. Oleate and linoleate were the dominant cellular fatty acids.

Author Keywords: Yarrowia lipolytica; Industrial glycerol; Lipid accumulation; Microbial lipids

Corresponding author. Tel.: +30-1529-4341; fax: +30-1529-4344; email: [George].[Aggelis]@[aua].[gr] - remove the [] to get the email address

Original source here from Science Direct

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